Recombinant Human IGF2

Insulin-like growth factor 2 (IGF2) is one of the mitogenic, polypeptide growth factors that belongs to the insulin family, due to its structural and functional similarity to insulin. IGF2 is mainly produced in the liver and several other tissues at certain times. IGF2 expression can be found in many tissues usually with autocrine, paracrine and endocrine functions. IGF2 mainly acts as a growth promoting hormone during gestation, and does so by binding to the tyrosine kinase type I receptor (IGF1R) as well as the IGF2 receptor cation-independent mannose 6-phosphate receptor. Mature IGF2 is highly conserved between human, bovine, and porcine species. Recombinant Human IGF2 is a single, non-glycosylated, polypeptide chain of 7.8 kDa.

Product Specifications

CAS Number

9000-83-3

Gene Name

IGF2

Gene Aliases

Insulin-like growth factor II, multiplication stimulating activity, MSA, Somatomedin-A, T3M-11-derived growth factor, PP1446

Gene ID

3481

Accession Number

P01344

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

Reconstitute in sterile PBS at 100 µg/mL

Function

The ED50 as determined by dose dependent proliferation of MCF7 cells was <1ng/ml

Molecular Weight

7.8

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.