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Recombinant Human GDNF

GDNF is a disulfide-bonded homodimeric glycosylated protein of 134 amino acids. The amino acid sequences inferred from rat and human cDNAs are 93% identical. The proteins contain seven conserved cysteine residues in the same relative spacing found in all members of the TGF-beta superfamily of proteins. In embryonic midbrain cultures GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Product Specifications

CAS Number

9000-83-3

Gene Name

GDNF

Gene Aliases

GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF<br/>Recombinant Human Glial-Derived Neurotrophic Factor (GDNF)

Accession Number

P39905

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED(50) was determined by the proliferation of rat C6 cells is &lt; 0.1 ng/mL, corresponding to a specific activity of &gt; 1.0 x 10^7 units/mg.

Molecular Weight

15

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Appearance

Lyophilized Powder

Formulation

Recombinant Glial-Derived Neurotrophic Factor was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.

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