Recombinant Human FLT3 Ligand (FLT3LG)

Known to bind to cells that express the tyrosine kinase receptor, FLT3LG promotes long-term expansion and differentiation of human pro-B cells. Human FLT3LG also stimulates the proliferation of cells expressing murine FLT-3 receptors. In combination with SCF and IL3, FLT3LG can cause expansion of cells with the marker spectrum CD34(+)/CD38(-). Alone, FLT3LG supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU-GEMM, and the very primitive high proliferative potential colony-forming cells, HPP-CFC. FLT3LG has only marginal effects on progenitors for erythroid cells and megakaryocytes. Recombinant Human FLT3LG is a non-glycosylated, monomeric protein with intra-chain disulfide bonds.

Product Specifications

CAS Number

9000-83-3

Gene Name

FLT3LG

Gene Aliases

Fms-related tyrosine kinase 3 ligand, FLT3 ligand, FLT3LG, FLT3L

Gene ID

2323

Accession Number

P49771

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED50 as determined by the dose-dependent stimulation of the proliferation of Human AML5 cells was found to be in the range of 1.0 ng/mL

Molecular Weight

17.8

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.