Recombinant Human FGF23

FGF-23 is a protein of ∼30 kDa that is proteolytically processed to generate smaller N-terminal (18 kDa) and C-terminal (12 kDa) fragments. The N-terminal fragment of FGF-23 contains the FGF receptor (FGFR)-binding domain. In vivo studies using synthetic peptides have indicated that neither of the processed N-terminal or C-terminal fragments of FGF-23 can influence serum phosphate levels. FGF-23 can decrease the activity of NaPi-2a and NaPi-2c cotransporters to reduce renal phosphate reabsorption and thereby can increase urinary phosphate excretion. Similarly, FGF-23 can suppress the expression of 1-alpha-hydroxylase to reduce the production of the active vitamin D metabolite 1,25(OH)2D. Moreover, FGF-23 can also induce 24- hydroxylase, which degrades 1,25(OH)2D

Product Specifications

CAS Number

9000-83-3

Gene Name

FGF23

Gene Aliases

FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor, HYPF<br/>Recombinant Human Fibroblast Growth Factor 23 (FGF23)

Gene ID

8074

Accession Number

Q9GZV9

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED(50) was determined by the dose-dependent proliferation of mouse 3T3 cells expressing FGF receptors, and was found to be < 0.3 ug/mL, corresponding to a specific activity of 3.0 x 10^4 units/mg.

Molecular Weight

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.