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Recombinant Human FGF19

Human FGF19 is expressed during brain development and embryogenesis and is a high-affinity, heparin-dependent ligand for FGF receptor 4. Among FGF family members, human FGF19 is most closely related to murine FGF15 and chicken FGF19. Recombinant Human FGF19 is a non-glycosylated, 21.9 kDa protein that contains 195 amino acid residues.

Product Specifications

Gene Name

FGF19

Gene Aliases

Fibroblast Growth Factor-19, FGFJ

Gene ID

9965

Accession Number

O95750

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml

Molecular Weight

21.9

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Appearance

Lyophilized Powder

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS

CAS Number

9000-83-3

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