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Recombinant Human DHH

The Desert Hedgehog protein is one of the three Hedgehog family proteins that is highly conserved and plays a role in many developmental processes in animals. DHH become biologically active following its autocatalytic cleavage. There is a high degree of residue homology between Sonic Hedgehog (SHH), Indian Hedgehog (IHH), and DHH. Despite their individual levels of expression and unique biological roles, the Hedgehog family members all use the same signalling pathway, and therefore, may be used interchangeably for some experimental purposes.

Product Specifications

CAS Number

9000-83-3

Gene Name

DHH

Gene Aliases

Desert Hedgehog Protein, DHH<br/>Recombinant Human Desert Hedgehog (DHH)

Gene ID

50846

Accession Number

O43323

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

Reconstitute at 500 μg/mL in sterile PBS

Function

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 15­-45 µg/mL

Molecular Weight

20

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Appearance

Lyophilized Powder

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 0.2 mM DTT

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