Recombinant Human Chemerin

Human Chemerin, also known as Tazarotene induced Gene 2 (TIG2), is an adipokine that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1. Its other ligands include G protein-coupled receptor 1 and chemokine receptor-like 2. Chemerin is expressed in various tissues where its biologically active form is generated by proteolytic removal of C-terminal residues by circulating proteases. In human, the soluble protoform gets processed into a monomeric, heparin binding bioactive molecule that can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases.

Product Specifications

CAS Number

9000-83-3

Gene Name

RARRES2

Gene Aliases

RAR-responsive protein TIG2, Tazarotene-induced gene 2 protein, Retinoic acid receptor responder protein 2, RARRES2

Gene ID

5919

Accession Number

Q99969

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human ChemR23. The ED(50) for this effect is typically 4 - 20 ng/mL.

Molecular Weight

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.