Recombinant Human AREG

Amphiregulin is a trans-membrane protein whose protease mediated digestion of the extracellular domain enables release of mature AR. Amphiregulin works to stimulate the proliferation of some animal keratinocyles, epithelial cells and fibroblasts. It has been shown that the mRNA expression of amphiregulin can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues, including colon, stomach, breast, ovary and kidney.

Product Specifications

CAS Number

9000-83-3

Gene Name

AREG

Gene Aliases

AR, Colorectum cell-derived growth factor, CRDGF<br/>Recombinant Human Amphiregulin (AREG)

Gene ID

374

Accession Number

P15514

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin

Function

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED(50) for this effect is typically 515 ng/mL.

Molecular Weight

11.5

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.