OrgFrontier™ Peroxisome Isolation Kit

Peroxisomes are small, single membrane-bound organelles that are found in all eukaryotic cells and are similar to lysosomes in size and morphology. Peroxisomes contain a variety of enzymes that utilize molecular oxygen to perform oxidation reactions. Peroxisomal enzymes are involved in the oxidation of ethanol, long-chain fatty acids, branched chain fatty acids, D-amino acids, and polyamines. Hydrogen peroxide, a product of the oxidative enzymatic reaction is subsequently converted to water and oxygen by catalase, an abundant peroxisomal enzyme. Peroxisomes are also involved in the biosynthesis of ether phospholipids known as plasmalogens that are required for the myelination of neurons. BioVision’s OrgFrontier™ Peroxisome Isolation Kit provides a proprietary set of reagents and buffers designed to enable the extraction and isolation of intact peroxisomes from mammalian tissues and cultured cells. The protocol requires an ultracentrifugation step and can be completed in one day.