Anti-Cavbeta2 Ca+2 Channel Antibody

Mouse Monoclonal Antibody specific to Cavß Ca+2 Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CAB2, Calcium channel voltage-dependent subunit beta 2

Gene Name

Cacnb2

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Cacnb2">Cacnb2</a>

UniProt

Q8VGC3

Accession Number

NP_446303

Cellular Locus

Cell membrane, sarcolemma, Peripheral membrane protein, Cytoplasmic side

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 189-205 of rat Cavb2 (RSPKPSANSVTSPHSKE) (accession no. NP_446303).

Target Antigen

Voltage-dependent L-type calcium channel subunit beta-2

Target

Cavbeta2 Ca+2 Channel

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, AM

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. {PubMed:11604404, PubMed:12042350, PubMed:1370480}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~78kDa is detected. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application. <br><br>Positive control: Rat brain lysate

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Cavb2. It does not cross-react with Cav b1, Cav b3, or Cav b4.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cavbeta subunits are involved in the transport of the pore-forming alpha1 subunit to the plasma membrane. They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore- forming subunit to the target membrane.