Biotinylated Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc, Avitag™ (MALS verified)

Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV) .

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 64.1 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q92692-2

Expression Region

Gln 32 - Leu 360

Host

HEK293

Target

Nectin-2

Conjugation

Biotin-labeled

Tag

C-mFc (mIgG2a) & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human PVRIG, His Tag (Cat. No. PVG-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) with a linear range of 0.6-10 ng/mL (QC tested).