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Anti-Mouse IgG2c Antibody, Alkaline Phosphatase conjugate

Goat Polyclonal Antibody specific to Mouse IgG2c-Alkaline Phosphatase Conjugate

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Mouse IgG2c-Alkaline Phosphatase Conjugate Goat Polyclonal Antibody

Host

Goat

Reactivity

Mouse

Immunogen

Mouse IgG2c

Target

Mouse IgG2c

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Secondary Antibodies

Purification Method

Purified by affinity chromatography on mouse IgG2c

Concentration

0.5 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

HEPES

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000-1:10,000. <br><br>Immunohistochemistry: use at a dilution of 1:100-1:1,000 <br><br>Immunocytochemistry: use at a dilution of 1:100-1:1,000 <br><br>ELISA: use at a dilution of 1:1,000-1:10,000. <br><br>These are recommended dilutions. <br><br>End users should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.

Storage Conditions

This antibody is stable for at least 1 year at 2-8°C.

Specificity

Antibody is affinity-purified on mouse IgG2c. By immunoblotting and ELISA, this antibody reacts specifically with IgG2c of C57Bl/6, SJL, C57BL/10, CB20, and C57BL/6 x Balb/c mice. Cross-reactivity with other mouse IgG subtypes is not detected.

Formulation

50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3

Buffer pH

pH 7.1

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