Anti-Mouse IgG2c Antibody, Alkaline Phosphatase conjugate
Goat Polyclonal Antibody specific to Mouse IgG2c-Alkaline Phosphatase Conjugate
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-Mouse IgG2c-Alkaline Phosphatase Conjugate Goat Polyclonal Antibody
Host
Goat
Reactivity
Mouse
Immunogen
Mouse IgG2c
Target
Mouse IgG2c
Clonality
Polyclonal
Isotype
Whole IgG
Type
Antibody
Applications
WB, IHC, ICC, ELISA
Field of Research
Secondary Antibodies
Purification Method
Purified by affinity chromatography on mouse IgG2c
Concentration
0.5 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
HEPES
Additionnal Information
Immunoblotting: use at a dilution of 1:1,000-1:10,000.
<br><br>Immunohistochemistry: use at a dilution of 1:100-1:1,000
<br><br>Immunocytochemistry: use at a dilution of 1:100-1:1,000
<br><br>ELISA: use at a dilution of 1:1,000-1:10,000.
<br><br>These are recommended dilutions.
<br><br>End users should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.
Storage Conditions
This antibody is stable for at least 1 year at 2-8°C.
Specificity
Antibody is affinity-purified on mouse IgG2c. By immunoblotting and ELISA, this antibody reacts specifically with IgG2c of C57Bl/6, SJL, C57BL/10, CB20, and C57BL/6 x Balb/c mice. Cross-reactivity with other mouse IgG subtypes is not detected.
Formulation
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3
Buffer pH
pH 7.1
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