Monkeypox virus (Democratic Republic of the Congo) M1R Protein, His Tag

Monkeypox virus (Democratic Republic of the Congo) M1R Protein, His Tag (M1R-M52H3) is expressed from human 293 cells (HEK293) . It contains AA Gly 2 - Gly 183 (Accession # EPI_ISL_19093812, GISAID) .

Product Specifications

Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. M1R promotes virus assembly.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.2 kDa. The protein migrates as 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

EPI_ISL_19093812

Expression Region

Gly 2 - Gly 183

Host

HEK293

Target

M1R (MPXV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Monkeypox virus (Democratic Republic of the Congo)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Monkeypox virus (Democratic Republic of the Congo) M1R Protein, His Tag (Cat. No. M1R-M52H3) at 1 μg/mL (100 μL/well) can bind Anti-Monkeypox virus/MPXV M1R Polyclonal Antibody with a linear range of 0.2-8 ng/mL (QC tested).