NuMA (A73-B/D12), 0.2mg/mL
Recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA) . Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.
Product Specifications
CAS Number
9007-83-4
Synonyms
Nuclear Mitotic Apparatus Protein 1; NuMA protein; NUMA1; SP-H antigen; Structural nuclear protein
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
NUMA1
Gene ID
4926
NCBI Gene ID
325978
UniProt
Q14980
Swiss Prot
Q14980
Host
Mouse
Species Reactivity
Human
Reactivity
NuMA
Immunogen
Colon carcinoma 174T cells
Target Antigen
NuMA
Clonality
Monoclonal
Clone
A73-B/D12
Type
Primary
Source
Animal
Origin
Animal
Applications
IHC, FFPE (verified)
Field of Research
Cancer, Cell cycle
Positive Control
Exponentially growing any cultured human cells. Tonsil or lymph node.
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
228 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgM, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA
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