Anti-ASC Antibody

Rabbit Polyclonal Antibody specific to ASC

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

hASC, Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1

Gene Name

PYCARD

Gene ID

29108

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=PYCARD">PYCARD</a>

UniProt

Q9ULZ3

Accession Number

NP_037390

Cellular Locus

Cytoplasm, Inflammasome, Endoplasmic reticulum, Mitochondrion, Nucleus

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 182-195 of human ASC.

Target Antigen

Apoptosis-associated speck-like protein containing a CARD

Target

ASC

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IHC-P

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern rPubMed:11103777, PubMed:12486103, PubMed:12646168, PubMed:14499617, PubMed:14730312, PubMed:15030775, PubMed:16585594, PubMed:16964285, PubMed:16982856, PubMed:17349957, PubMed:17599095, PubMed:19158675, PubMed:19158676, PubMed:19234215, PubMed:19494289, PubMed:21487011, PubMed:22732093, PubMed:28314590, PubMed:30674671, PubMed:34678144}.; [Isoform 2]: May have a regulating effect on the function as inflammasome adapter. {PubMed:19759850, PubMed:20482797}.; [Isoform 3]: Seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. {PubMed:20482797}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate of HL60 cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human ASC (25kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel CARD-containing protein was recently identified and designated ASC (or TMS1). ASC induces apoptosis by activating caspase-9 and inhibits survival of human breast cancer cells . ASC is widely expressed in human and mouse tissues.