HEPES Ultra Pure

<strong>HEPES Ultra Pure_x000D_ </strong>_x000D_ <table width="1028">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="285">Catalog number:</td>_x000D_ <td width="743">B2010032U</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume/Weight</td>_x000D_ <td>50 g</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Molecular Formula</td>_x000D_ <td>C8H18N2O4S</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Molecular Weight</td>_x000D_ <td>238.3</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>CAS Number</td>_x000D_ <td>7365-45-9</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Application:</td>_x000D_ <td>buffer formulation, reagent formulation, reagent stabilization</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>2 years from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Room temperature</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="743">1: Morgia F, Torti M, Montigiani M, Piscitelli C, Giallonardo A, Schimberni M,_x000D_ Giannini P, Sbracia M. Use of a medium buffered with N-hydroxyethylpiperazine-N-_x000D_ ethanesulfonate (HEPES) in intracytoplasmic sperm injection procedures is_x000D_ detrimental to the outcome of in vitro fertilization. Fertil Steril. 2006_x000D_ May;85(5):1415-9._x000D_ 2: Simpson JA, Cheeseman KH, Smith SE, Dean RT. Free-radical generation by_x000D_ copper ions and hydrogen peroxide. Stimulation by Hepes buffer. Biochem J. 1988_x000D_ Sep 1;254(2):519-23.3: Kasraian K, Kuzniar A, Earley D, Kamicker BJ, Wilson G, Manion T, Hong J,_x000D_ Reiber C, Canning P. Sustained in vivo activity of recombinant bovine_x000D_ granulocyte colony stimulating factor (rbG-CSF) using HEPES buffer. Pharm Dev_x000D_ Technol. 2001 Aug;6(3):441-7.4: Habib A, Tabata M. Oxidative DNA damage induced by HEPES_x000D_ (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) buffer in the presence_x000D_ of Au(III). J Inorg Biochem. 2004 Nov;98(11):1696-702._x000D_ _x000D_ 5: Vasconcelos MT, Azenha MA, Lage OM. Electrochemical evidence of surfactant_x000D_ activity of the hepes pH buffer which may have implications on trace metal_x000D_ availability to cultures in vitro. Anal Biochem. 1996 Oct 15;241(2):248-53._x000D_ _x000D_ 6: Lomonosova EE, Kirsch M, Rauen U, de Groot H. The critical role of Hepes in_x000D_ SIN-1 cytotoxicity, peroxynitrite versus hydrogen peroxide. Free Radic Biol Med._x000D_ 1998 Mar 1;24(4):522-8._x000D_ _x000D_ 7: Kirsch M, Lomonosova EE, Korth HG, Sustmann R, de Groot H. Hydrogen peroxide_x000D_ formation by reaction of peroxynitrite with HEPES and related tertiary amines._x000D_ Implications for a general mechanism. J Biol Chem. 1998 May 22;273(21):12716-24._x000D_ _x000D_ 8: Cowan AI, Martin RL. Simultaneous measurement of pH and membrane potential in_x000D_ rat dorsal vagal motoneurons during normoxia and hypoxia: a comparison in_x000D_ bicarbonate and HEPES buffers. J Neurophysiol. 1995 Dec;74(6):2713-21._x000D_ _x000D_ 9: Svajger U, Jeras M. Optimal dendritic cell differentiation in rpmi media_x000D_ requires the absence of HEPES buffer. <a href="https://pubmed.ncbi.nlm.nih.gov/21314286/">Immunol Invest.</a> 2011;40(4):413-26.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Related Products</td>_x000D_ <td><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>