T7 RNA Polymerase (Highly Purified)

<strong>T7 RNA Polymerase</strong>_x000D_ <strong>Catalog number:</strong> B2010405_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 5000 Units_x000D_ <strong>Molecular Weight or Concentration:</strong> 99 kDa_x000D_ <strong>Supplied as:</strong> Liquid Solution_x000D_ <strong>Appearance:</strong> Clear Liquid_x000D_ <strong>Applications:</strong> Highly purified and highly specific Bacteriophase T7 RNA Polymerase. This enzyme was expressed in E. coli and is provided with a 10X reaction buffer._x000D_ <strong>Storage:</strong> -20C_x000D_ <strong>Keywords:</strong> T7 RNAP_x000D_ <strong>Grade:</strong> Biotechnology grade. All solid components are highly pure (minimum 95%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Tabor S. Expression using the T7 RNA polymerase/promoter system. Curr Protoc_x000D_ Mol Biol. 2001 May;Chapter 16:Unit16.2._x000D_ _x000D_ 2: Borkotoky S, Murali A. The highly efficient T7 RNA polymerase: A wonder_x000D_ macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt_x000D_ A):49-56._x000D_ _x000D_ 3: Passalacqua LFM, Dingilian AI, Lupták A. Single-pass transcription by T7 RNA_x000D_ polymerase. RNA. 2020 Dec;26(12):2062-2071._x000D_ _x000D_ 4: Kar S, Ellington AD. Construction of synthetic T7_RNA polymerase expression_x000D_ systems. Methods. 2018 Jul 1;143:110-120._x000D_ _x000D_ 5: Potapov V, Fu X, Dai N, Corrêa IR Jr, Tanner NA, Ong JL. Base modifications_x000D_ affecting RNA polymerase and reverse transcriptase fidelity. Nucleic Acids Res._x000D_ 2018 Jun 20;46(11):5753-5763._x000D_ _x000D_ 6: Macdonald LE, Durbin RK, Dunn JJ, McAllister WT. Characterization of two_x000D_ types of termination signal for bacteriophage T7_RNA polymerase. J Mol Biol._x000D_ 1994 Apr 29;238(2):145-58._x000D_ _x000D_ 7: Zhang X, Yao Z, Duan Y, Zhang X, Shi J, Xu Z. Investigation of specific_x000D_ interactions between T7 promoter and T7_RNA polymerase by force spectroscopy_x000D_ using atomic force microscope. Biochem J. 2018 Jan 11;475(1):319-328._x000D_ _x000D_ 8: Gao W , Xu J , Lian G , Wang X , Gong X , Zhou D , Chang J . A novel_x000D_ analytical principle using AP site-mediated T7_RNA polymerase transcription_x000D_ regulation for sensing uracil-DNA glycosylase activity. Analyst. 2020 Jun_x000D_ 21;145(12):4321-4327._x000D_ _x000D_ 9: Ji S, Thomforde J, Rogers C, Fu I, Broyde S, Tretyakova NY. Transcriptional_x000D_ Bypass of DNA-Protein and DNA-Peptide Conjugates by T7_RNA Polymerase. ACS Chem_x000D_ Biol. 2019 Dec 20;14(12):2564-2575._x000D_ _x000D_ 10: Juhas M, Ajioka JW. T7 RNA_polymerase-driven inducible cell lysis for DNA_x000D_ transfer from Escherichia coli to Bacillus subtilis. Microb Biotechnol. 2017_x000D_ Nov;10(6):1797-1808._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/30219859/">11: Gholamalipour Y, Karunanayake Mudiyanselage A, Martin CT. 3' end additions_x000D_ by T7 RNA polymerase are RNA self-templated, distributive and diverse in_x000D_ character-RNA-Seq analyses. Nucleic Acids Res. 2018 Oct 12;46(18):9253-9263.</a>_x000D_ _x000D_ <strong>Products Related to T7 RNA Polymerase </strong> <a href="https://moleculardepot.com/product-category/Enzymes/">: Enzymes</a>