Anti-Sodium Iodide Symporter Antibody

Mouse Monoclonal Antibody specific to Sodium Iodine Symporter

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Na(+/I(- cotransporter, Sodium-iodide symporter, Na(+/I(- symporter, Solute carrier family 5 member 5

Gene Name

SLC5A5

NCBI Gene ID

UniProt

Q92911

Accession Number

NP_000444.1

Cellular Locus

Membrane; Multi-pass membrane protein.

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein consisting of aa468-643 of human NIS attached to mannose binding protein (MBP).

Target Antigen

Sodium/iodide cotransporter

Target

Sodium Iodide Symporter

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, AM

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Mediates iodide uptake in the thyroid gland.

Additionnal Information

Immunoblotting: use at 1ug/mL. A major band of ~97kDa is detected. Depending on the sample, some minor bands may be detected as well.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse and rat NIS.Immunohistochemistry: use at 1-10ug/ml. Positive control: Graves disease-thyroid tissue.These are recommended concentrations; enduser should determine optimal concentrations for their

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells. This is an important step in the process of iodide organification and the formation of triiodothyronine and thyroxine. The importance of NIS is related to its role in thyroid function, the pathogenesis of thyroid disease, and its expression (or lack of expression) in thyroid malignancies. It has also been suggested that NIS may represent an autoantigen in autoimmune disease of the thyroid.