Vinculin Monoclonal Antibody
Product Specifications
Background
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin) -binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Abbreviation
Vinculin
UniProt
P18206
Host
Mouse
Reactivity
Human; Mouse; Rat
Immunogen
Recombinant human Vinculin protein expressed by E.coli
Target
Vinculin; Metavinculin (MV) ; VCL
Clonality
Monoclonal
Clone
4D4
Conjugation
Unconjugated
Applications
WB; IHC; IF
Field of Research
Cardiovascular; Signal Transduction; Isotype/Loading Controls; Epigenetics and Nuclear Signaling
Purification
Protein A/G Purification
Concentration
1 mg/mL
Dilution
WB 1:2000-1:4000; IHC 1:200-1:400; IF 1:200-1:400
Buffer
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Calculated Molecular Weight
124 kDa
Observed Molecular Weight
126 kDa
Isotype
IgG1
Available Sizes
Documents
More Discoveries
Explore Other Products
Browse additional items from our catalog
SR320715 1 Kit
Integrin beta 8 (ITGB8) Human siRNA Oligo Duplex (Locus ID 3696)
Sign In for Pricing
View Details 
MBS6466660-02 5x 0.2 mL
CALD1, ID (Caldesmon, CDM, CAD, CALD1) (FITC)
Sign In for Pricing
View Details