Vinculin Monoclonal Antibody
Product Specifications
Background
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin) -binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Abbreviation
Vinculin
UniProt
P18206
Host
Mouse
Reactivity
Human; Mouse; Rat
Immunogen
Recombinant human Vinculin protein expressed by E.coli
Target
Vinculin; Metavinculin (MV) ; VCL
Clonality
Monoclonal
Clone
4D4
Conjugation
Unconjugated
Applications
WB; IHC; IF
Field of Research
Cardiovascular; Signal Transduction; Isotype/Loading Controls; Epigenetics and Nuclear Signaling
Purification
Protein A/G Purification
Concentration
1 mg/mL
Dilution
WB 1:2000-1:4000; IHC 1:200-1:400; IF 1:200-1:400
Buffer
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Calculated Molecular Weight
124 kDa
Observed Molecular Weight
126 kDa
Isotype
IgG1
Available Sizes
Documents
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