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Vinculin Monoclonal Antibody

Product Specifications

Background

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin) -binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Abbreviation

Vinculin

UniProt

P18206

Host

Mouse

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant human Vinculin protein expressed by E.coli

Target

Vinculin; Metavinculin (MV) ; VCL

Clonality

Monoclonal

Clone

4D4

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Cardiovascular; Signal Transduction; Isotype/Loading Controls; Epigenetics and Nuclear Signaling

Purification

Protein A/G Purification

Concentration

1 mg/mL

Dilution

WB 1:2000-1:4000; IHC 1:200-1:400; IF 1:200-1:400

Buffer

PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Product Datasheet

https://789.bio/ea/dquwsr

Product MSDS

https://789.bio/eb/4ZPxWH

Calculated Molecular Weight

124 kDa

Observed Molecular Weight

126 kDa

Isotype

IgG1

Available Sizes

Curated Selection

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