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Mucin 1 / EMA / Episialin / CD227 (E29), 0.2mg/mL

In Western blotting, this antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

CAS Number

9007-83-4

Synonyms

Breast carcinoma-associated antigen DF3; CA15-3; Carcinoma-associated mucin Episialin; Epithelial Membrane Antigen; H23AG; KL-6; MAM6; MUC-1; MUC-1/SEC; MUC-1/X; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; Mucin 1 cell surface associated; Mucin-1 subunit beta; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

MUC1

Gene ID

4582

NCBI Gene ID

89603

UniProt

P15941

Swiss Prot

P15941

Host

Mouse

Species Reactivity

Human

Reactivity

CD227|EMA|Episialin|Mucin 1

Immunogen

Delipidated extract of human milk fat globule membranes

Target Antigen

CD227 | EMA | Episialin | Mucin 1

Clonality

Monoclonal

Clone

E29

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Field of Research

Cancer, Mucins

Positive Control

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

265-400 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA

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