Anti-Endothelin Antibody
Mouse Monoclonal Antibody specific to Endothelin
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Preproendothelin-1, PPET1 [Cleaved into: Endothelin-1
Gene Name
EDN1
NCBI Gene ID
<a href="https://www.ncbi.nlm.nih.gov/gene/?term=EDN1">EDN1</a>
UniProt
P05305
Cellular Locus
Secreted.
Host
Mouse
Reactivity
Human
Immunogen
Human endothelin-1 (ET-1)
Target Antigen
Endothelin-1
Target
Endothelin
Clonality
Monoclonal
Isotype
IgG1
Type
Antibody
Applications
ELISA, WB, IHC
Field of Research
Hormones/Proteins
Purification Method
Purified by Protein G affinity chromatography
Concentration
Lot Specific
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Function
Endothelins are endothelium-derived vasoconstrictor peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed:19086031). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437). {UniProtKB:P09558, PubMed:17446437, PubMed:19086031}.
Additionnal Information
<br><br>Immunohistochemistry:Paraffin-embedded rat kidney exposed to 60 minutes of ischemia. ET-1 expression is seen in the endothelium of the peritubular capillary network (arrows, dark reaction product) with #19401 at 5-20ug/ml. Wilhelm SM et al. 1999 Kidney International 55: 1011-1018.ELISA:use at 5-20ug/ml with endothelin-1 on the solid phase.
<br><br>These are recommended concentrations.
<br><br>Endusers should determine optimal concentrations for their applications.
Storage Conditions
These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Specificity
Human endothelin-1 (ET-1).
Formulation
PBS, pH 7.4.
Buffer pH
pH 7.4
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