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MHC II (HLA-DP) (beta chain) (BRA-FB6), 0.2mg/mL

Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linkedalpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

CAS Number

9007-83-4

Synonyms

Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

HLA-DPB1

Gene ID

3115

NCBI Gene ID

347270

UniProt

P04440

Swiss Prot

P04440

Host

Mouse

Species Reactivity

Human

Reactivity

MHC-II

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Target Antigen

MHC-II

Clonality

Monoclonal

Clone

BRA-FB6

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |Flow, surface (published) |Functional studies (published) |ELISA (published) |IP (published)

Field of Research

Immunology, MHC antigens

Positive Control

Raji cells. Tonsil or lymph node

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

33-35 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Tissue Antigens (1988) 32(1):6-11. (IP) Cancer Sci (2008) 99(8): 1633-1642. (ELISPOT) World J Gastroenterol (2003) 9(5): 1077-1081. (Flow, surface) Eur J Immunol (2009) 39: 1947-1955. (functional studies)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA

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