Melanoma Marker (HMB45 + A103 + T311), 0.2mg/mL
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Specifications
CAS Number
9007-83-4
Synonyms
Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; TYR; PMEL17
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene ID
2315-MART-1; 7299-Tyrosinase; 6490-gp100
NCBI Gene ID
154069-MART-1; 503555-Tyrosinase; 95972-gp100
UniProt
Q16655-MART-1; P14679-Tyrosinase; P40967-gp100
Swiss Prot
Q16655-MART-1; P14679-Tyrosinase; P40967-gp100
Host
Mouse
Species Reactivity
Human
Reactivity
Melanoma Marker
Immunogen
Recombinant hMART-1 protein (A103) ; Recombinant tyrosinase protein (T311) ; Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Target Antigen
Melanoma Marker
Clonality
Monoclonal
Clone
HMB45 A103 T311
Type
Primary
Source
Animal
Origin
Animal
Applications
IHC, FFPE (verified) |WB (verified)
Field of Research
Cancer
Positive Control
SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
20-22 kDa (doublet) (MART) ; 70-80 kDa (Tyrosinase) ; 90-100 kDa (gp100)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG' s
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA
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