Ku-Holo (p70;83) (KU729), 0.2mg/mL

Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

CAS Number

9007-83-4

Synonyms

Ku (p70) : 70kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70kDa subunit; CTC box-binding factor 75kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen, 70kDa; Ku70; Kup70; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen) ; Thyroid-lupus autoantigen (TLAA) ; X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80) : 86kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 86Kd subunit; ATP-dependent DNA helicase II 80kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV) ; Thyroid-lupus autoantigen (TLAA) ; X-ray repair cross-complementing protein 5 (XRCC5)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

XRCC6

Gene ID

2547 (p70); 7520 (p80)

NCBI Gene ID

292493 (p70); 388739 (p80)

UniProt

P12956 (p70); P13010 (p80)

Swiss Prot

P12956 (p70) ; P13010 (p80)

Host

Mouse

Species Reactivity

Human,Non-human primates

Reactivity

Ku-Holo

Immunogen

Nuclear extract of human HL-60 cells

Target Antigen

Ku-Holo

Clonality

Monoclonal

Clone

KU729

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (verified) |IF (verified)

Field of Research

Apoptosis, Cell cycle, Signal transduction

Positive Control

Human cancer K562, HL-60, HEp-2 or HeLa cells

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

70 kDa & 80 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA

Documents

Product Datasheet

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Product MSDS

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