Human alpha-Thrombin with Blocked DFP Active Site

<strong>Human alpha-Thrombin with Blocked DFP Active Site</strong>_x000D_ <strong>Catalog number:</strong> B2016614_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 50 µg_x000D_ <strong>Molecular Weight or Concentration:</strong> 36700_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> Human alpha-Thrombin, DFP active site blocked_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong> _x000D_ 1: Sekine O, Sugo T, Ebisawa K, Umeyama H, Iwahana H, Ruiz-Sáez A, de Bosch N, Matsudai M. Substitution of Gly-548 to Ala in the substrate binding pocket of prothrombin Perijá leads to the loss of thrombin proteolytic activity Thromb Haemost. 2002 Feb;87(2):282-7._x000D_ 2: Fenton JW 2nd, Bing DH. Thrombin active-site regions Semin Thromb Hemost. 1986 Jul;12(3):200-8. _x000D_ 3: Skrzypek E, Straley SC. Interaction between Yersinia pestis YopM protein and human alpha-thrombin Thromb Res. 1996 Oct 1;84(1):33-43. _x000D_ 4: Seiler SM. Thrombin receptor antagonists Semin Thromb Hemost. 1996;22(3):223-32. _x000D_ 5: Esmon CT. Regulation of blood coagulation Biochim Biophys Acta. 2000 Mar 7;1477(1-2):349-60. _x000D_ 6: Woodruff RS, Sullenger BA. Modulation of the Coagulation Cascade Using Aptamers Arterioscler Thromb Vasc Biol. 2015 Oct;35(10):2083-91. _x000D_ 7: Ye J, Esmon NL, Esmon CT, Johnson AE. The active site of thrombin is altered upon binding to thrombomodulin. Two distinct structural changes are detected by fluorescence, but only one correlates with protein C activation J Biol Chem. 1991 Dec 5;266(34):23016-21._x000D_ 8: Rao LV, Ezban M. Active site-blocked activated factor VII as an effective antithrombotic agent: mechanism of action Blood Coagul Fibrinolysis. 2000 Apr;11 Suppl 1:S135-43. _x000D_ 9: Richardson JL, Fuentes-Prior P, Sadler JE, Huber R, Bode W. Characterization of the residues involved in the human alpha-thrombin-haemadin complex: an exosite II-binding inhibitor Biochemistry. 2002 Feb 26;41(8):2535-42. _x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/22982873">10: Coppens M, Eikelboom JW, Gustafsson D, Weitz JI, Hirsh J. Translational success stories: development of direct thrombin inhibitors Circ Res. 2012 Sep 14;111(7):920-9. </a>_x000D_ <br><strong>Products Related to Human alpha-Thrombin with Blocked DFP Active Site can be found at</strong> <a href="https://moleculardepot.com/product-category/Proteins/"> Proteins</a>