Anti-DNA Ligase I Antibody
Mouse Monoclonal Antibody specific to DNA Ligase I
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
EC 6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1
Gene Name
LIG1
NCBI Gene ID
<a href="https://www.ncbi.nlm.nih.gov/gene/?term=LIG1">LIG1</a>
UniProt
P18858
Cellular Locus
Nucleus.
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.
Target Antigen
DNA ligase 1
Target
DNA Ligase I
Clonality
Monoclonal
Isotype
IgG1
Type
Antibody
Applications
WB, ICC/IF, IHC-P, IP
Field of Research
Cancer research
Purification Method
Purified by Protein G affinity chromatography
Concentration
Lot Specific
Dilution
Dilute in PBS or medium which is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Additionnal Information
Immunoblotting: use at 1-5 ug/mL. <br><br>Immunoprecipitation: use at 1-5 ug/mL. <br><br>Positive controls: MCF-7 cells or recombinant protein.
Storage Conditions
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Specificity
Formulation
PBS, pH 7.4
Buffer pH
pH 7.4
Frequently Asked Questions
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