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Anti-DNA Ligase I Antibody

Mouse Monoclonal Antibody specific to DNA Ligase I

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1

Gene Name

LIG1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=LIG1">LIG1</a>

UniProt

P18858

Cellular Locus

Nucleus.

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.

Target Antigen

DNA ligase 1

Target

DNA Ligase I

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IHC-P, IP

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Additionnal Information

Immunoblotting: use at 1-5 ug/mL. <br><br>Immunoprecipitation: use at 1-5 ug/mL. <br><br>Positive controls: MCF-7 cells or recombinant protein.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human DNA Ligase I (125 kD), one of the four ligase proteins found in mammalian cells that are required for the joining of DNA strand breaks that occur during DNA replication, repair, and recombination.

Formulation

PBS, pH 7.4

Buffer pH

pH 7.4

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