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Human Haptoglobin Protein, His tag

Haptoglobin is a glycoprotein, which is synthesized in the liver and circulates in the blood. Haptoglobin is usually produced by liver cells, but it is also produced by other tissues, such as skin, lung and kidney. It is a positive acute phase protein, which binds to free hemoglobin and removes it from circulation to prevent kidney injury and iron loss after hemolysis. Decreased levels can be seen in hemolysis and impaired liver function. A high level is a sign of acute or chronic inflammation. Hemoglobin deficiency or hypohaptoglobinemia is caused by mutation of haptoglobin gene and/or its regulatory region. Haptoglobin is also related to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, susceptibility to primary sclerosing cholangitis, idiopathic Parkinson's disease and the reduction of the incidence of plasmodium falciparum malaria.

Product Specifications

Background

Haptoglobin is a glycoprotein, which is synthesized in the liver and circulates in the blood. Haptoglobin is usually produced by liver cells, but it is also produced by other tissues, such as skin, lung and kidney. It is a positive acute phase protein, which binds to free hemoglobin and removes it from circulation to prevent kidney injury and iron loss after hemolysis. Decreased levels can be seen in hemolysis and impaired liver function. A high level is a sign of acute or chronic inflammation. Hemoglobin deficiency or hypohaptoglobinemia is caused by mutation of haptoglobin gene and/or its regulatory region. Haptoglobin is also related to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, susceptibility to primary sclerosing cholangitis, idiopathic Parkinson's disease and the reduction of the incidence of plasmodium falciparum malaria.

Product Name Alternative

Haptoglobin

Gene ID

3240

Accession Number

P00738

Tag

Haptoglobin

Type

Cytokines and proteins

Applications

His tag

Purity

> 95 % as determined by SDS-PAGE

Activity

Testing in progress

Molecular Weight

29.5 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized Haptoglobin protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant Human Haptoglobin consists of 245 amino acids and predicts a molecular mass of 29.5 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4.

Applications Notes

Human

Other Product Names

Haptoglobin

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Preparation

E. coli

Accession Number URL

https://www.uniprot.org/uniprotkb/P00738

AA Sequence

Amino acid sequence derived from Human Haptoglobin (AAA88080.1) (Ile162-Asn406) was expressed with 6xHis tag at N-terminus.

Available Sizes

Frequently Asked Questions

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