EGFR (B1D8), CF568 conjugate, 0.1mg/mL

This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGF induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, β cellulin and epiregulin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

CAS Number

9007-83-4

Synonyms

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

EGFR

Gene ID

1956

NCBI Gene ID

488293

UniProt

P00533

Swiss Prot

P00533

Host

Mouse

Species Reactivity

Human

Reactivity

EGFR

Immunogen

Microsomes from A431 cells.

Target Antigen

EGFR

Clonality

Monoclonal

Clone

B1D8

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published) |IP (published)

Field of Research

Cancer, Signal transduction

Positive Control

A431 cells, Breast or Bladder cancer

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

~170 kDa (wild type) and ~145 kDa (vIII variant)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1984) 259(19): 11895-11900. (IP, functional studies) J Immunol Meth (2004) 287: 147-158. (Flow; epitope mapping)