Recombinant Staphylococcus aureus Sortase A (srtA) , partial (P94R, D160N, D165A, K190E, K196T, D124G, Y187L, E189R)
Product Specifications
CAS Number
9000-83-3
Gene Name
srtA
UniProt
Q2FV99
Expression Region
25-206aa (P94R, D160N, D165A, K190E, K196T, D124G, Y187L, E189R)
Organism
Staphylococcus aureus (strain NCTC 8325)
Target Sequence
AKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATREQLNRGVSFAEENESLDDQNISIAGHTFIGRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRNVKPTAVGVLDEQKGKDKQLTLITCDDLNRETGVWETRKIFVATEVK
Tag
N-terminal 6xHis-tagged
Source
Yeast
Field of Research
Microbiology
Assay Type
Developed Protein
Relevance
Transpeptidase that anchors surface proteins to the cell wall . Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal.
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Length
Partial
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
22.0 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Available Sizes
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