Cytokeratin, HMW (AE-3), CF647 conjugate, 0.1mg/mL
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1) ; 64 kDa (CK3) ; 59 kDa (CK4) ; 58 kDa (CK5) ; 56 kDa (CK6) ; 52 kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Specifications
CAS Number
9007-83-4
Synonyms
KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P) ; Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
KRT76
Gene ID
51350
NCBI Gene ID
654392
UniProt
Q01546
Swiss Prot
Q01546
Host
Mouse
Species Reactivity
Chicken,Cow,Dog,Human,Monkey,Mouse,Rabbit,Rat
Reactivity
Cytokeratin, HMW
Immunogen
Human epidermal keratin
Target Antigen
Cytokeratin, HMW
Clonality
Monoclonal
Clone
AE-3
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, intracellular|IF (published) |IHC, frozen (published) |IHC, FFPE (verified) |WB (published)
Field of Research
Cancer, Cytoskeleton
Positive Control
Epithelial cells, Skin or Adenocarcinomas
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
52-67 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1982) 95: 580-588. (IHC, frozen; IF; WB)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Product Datasheet
https://cdn.gentaur.com/products/37/878581/datasheet/bnc470257-500.pdf
Product MSDS
https://cdn.gentaur.com/products/37/68dd02aa061270361e107734/msds/bnc470257-500.pdf
Fragment
IgG1, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF647
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
