Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VIP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
UniProt
P01283
Reactivity
Rat
Field of Research
Endocrinology; Gastroenterology; Hormone metabolism
Assay Type
Sandwich
Assay Principle
Assay Performance Time
3.5h
Standard
500 pg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.82-500 pg/mL
Sensitivity
2.7 pg/mL
Available Sizes
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