Rat NOS1 (Nitric Oxide Synthase 1, Neuronal) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
NOS; nNOS; IHPS1; NC-NOS; N-NOS; bNOS; Neuronal NOS; Constitutive NOS; NOS type I; Nitric Ocide Synthase; Peptidyl-cysteine S-nitrosylase NOS1
Gene Aliases
NOS; nNOS; IHPS1; NC-NOS; N-NOS; bNOS; Neuronal NOS; Constitutive NOS; NOS type I; Nitric Ocide Synthase; Peptidyl-cysteine S-nitrosylase NOS1
UniProt
P29476
Reactivity
Rat
Field of Research
Signal transduction, Enzyme & Kinase, Metabolic pathway, Neuro science
Assay Type
Sandwich
Assay Principle
Assay Performance Time
3.5 H
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
0.16 - 10 ng/mL
Sensitivity
0.053 ng/mL
Product Datasheet
https://www.elkbiotech.com/upload/file/ELISA/ELK1620-1.pdf
Species
Rat
CAS Number
7732-18-5
Available Sizes
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