Rat HSPB1 (Heat Shock 27kDa Protein 1) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPB1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
HSP27; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock Protein 27; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein
Gene Aliases
HSP27; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock Protein 27; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
UniProt
P42930
Reactivity
Rat
Field of Research
Signal transduction, Tumor immunity, Developmental science
Assay Type
Sandwich
Assay Principle
Assay Performance Time
3.5 H
Standard
50 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Detection Range
0.79 - 50 ng/mL
Sensitivity
0.27 ng/mL
Product Datasheet
https://www.elkbiotech.com/upload/file/ELISA/ELK1846-1.pdf
Species
Rat
CAS Number
7732-18-5
Available Sizes
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