Cytokeratin 10/13 (DE-K13), 0.2mg/mL
This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Specifications
CAS Number
9007-83-4
Synonyms
BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT1
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
KRT10
Gene ID
3858
NCBI Gene ID
99936
UniProt
P13645
Swiss Prot
P13645
Host
Mouse
Species Reactivity
Cat,Human
Reactivity
Cytokeratin 10/13
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium
Target Antigen
Cytokeratin 10/13
Clonality
Monoclonal
Clone
DE-K13
Type
Primary
Source
Animal
Origin
Animal
Applications
IF (verified) |IHC, FFPE (verified)
Field of Research
Cancer, Cytoskeleton
Positive Control
A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
56.5 kDa (CK10) & 53 kDa (CK13)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA
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