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Human GDF1 (Growth Differentiation Factor 1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GDF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GDF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

Embryonic growth/differentiation factor 1

Gene Aliases

Embryonic growth/differentiation factor 1

UniProt

P27539

Reactivity

Human

Field of Research

Cytokine, Neuro science, Developmental science, Genetic science

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GDF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GDF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5 H

Standard

10 ng/mL

Sample Type

Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection Range

0.16 - 10 ng/mL

Sensitivity

0.059 ng/mL

Product Datasheet

https://www.elkbiotech.com/upload/file/ELISA/ELK3061-1.pdf

Species

Human

CAS Number

7732-18-5

Available Sizes

Curated Selection

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