H2B (Histone H2B) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human,Rat,Mouse H2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human,Rat,Mouse H2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human,Rat,Mouse H2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human,Rat,Mouse H2B in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

HIST1H2BA; testis; bA317E16.3; H2B; H2B histone family member U; H2B histone family member U testis specific; H2B histone family, member U, (testis specific) ; H2B testis; H2B1A_HUMAN; H2BFU; H2BT; HIST1H2BA; Histone 1, H2ba; Histone cluster 1 H2ba; Histone H2B; Histone H2B testis; Histone H2B type 1 A; Histone H2B type 1-A; Histone H2B type 1A; STBP; Testis specific histone H2B; Testis-specific histone H2B; TSH 2B; TSH2B; TSH2B.1

Reactivity

Human, Rat, Mouse

Field of Research

Signal transduction; Developmental science

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human, Rat, Mouse H2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human, Rat, Mouse H2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human, Rat, Mouse H2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human, Rat, Mouse H2B in the samples is then determined by comparing the OD of the samples to the standard curve.