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Human TUBb (Tubulin Beta) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TUBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TUBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TUBb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TUBb in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

TUB-B; TUBB5; TUBB1; M40

Gene Aliases

TUB-B; TUBB5; TUBB1; M40

UniProt

P07437

Reactivity

Human

Field of Research

Neuro science, Developmental science

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TUBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TUBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TUBb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TUBb in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5 H

Standard

20 ng/mL

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

0.32 - 20 ng/mL

Sensitivity

0.115 ng/mL

Product Datasheet

https://www.elkbiotech.com/upload/file/ELISA/ELK5140-1.pdf

Species

Human

CAS Number

7732-18-5

Available Sizes

Curated Selection

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