Pig LIF (Leukemia Inhibitory Factor) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig LIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig LIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig LIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig LIF in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin
Reactivity
Pig
Field of Research
Cytokine; Tumor immunity; Infection immunity; Neuro science; Bone metabolism
Assay Type
Sandwich
Assay Principle
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
11.5 pg/mL
Available Sizes
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
KCNJ18 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
USF2, ID (Upstream Stimulatory Factor 2, Upstream Transcription Factor 2, FOS-interacting Protein, FIP, Major Late Transcription Factor 2, Class B Basic Helix-loop-helix Protein 12, bHLHb12) (AP) discontinued