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Mouse IL17C (Interleukin 17C) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL17C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL17C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL17C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL17C in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

IL-17C; Cytokine CX2

Gene Aliases

IL-17C;Cytokine CX2

UniProt

Q8K4C5

Reactivity

Mouse

Field of Research

Cytokine

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL17C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL17C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL17C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL17C in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5 H

Standard

1000 pg/mL

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

15.63 - 1000 pg/mL

Sensitivity

5.6 pg/mL

Product Datasheet

https://www.elkbiotech.com/upload/file/ELISA/ELK6078-1.pdf

Species

Mouse

CAS Number

7732-18-5

Available Sizes

Curated Selection

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