RT3 (Reverse Triiodothyronine) ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
RT3; r-T3; Reverse T3; (2S) -2-Amino-3-[4- (4-Hydroxy-3,5-Diiodophenoxy) -3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine
Gene Aliases
RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine
Reactivity
General
Field of Research
Infection immunity, Endocrinology, Hormone metabolism, Autoimmunity
Assay Type
Competitive Inhibition
Assay Principle
Assay Performance Time
2 H
Standard
6000 pg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
93.75 - 6000 pg/mL
Sensitivity
27.68 pg/mL
Product Datasheet
https://www.elkbiotech.com/upload/file/ELISA/ELK7898-1.pdf
Species
General
CAS Number
9000-83-3,7732-18-5
Available Sizes
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