ATP (Adenosine Triphosphate) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ATP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

Adenosine-5'-Triphosphate

Reactivity

General

Field of Research

Nutrition metabolism

Assay Type

Competitive Inhibition

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ATP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP in the samples is then determined by comparing the OD of the samples to the standard curve.