PAF (Platelet Activating Factor) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PAF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAF in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

PAF-Acether; AGEPC; Acetyl-Glyceryl-Ether-Phosphorylcholine

Reactivity

General

Field of Research

Metabolic pathway; Infection immunity; Cardiovascular biology

Assay Type

Competitive Inhibition

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PAF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAF in the samples is then determined by comparing the OD of the samples to the standard curve.