Cattle HB (Hemoglobin) ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HB in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
Hgb; Haemoglobin; Heterotetramer (αβ) 2
Reactivity
Cattle
Field of Research
Metabolic pathway; Infection immunity; Hematology; Neuro science
Assay Type
Competitive Inhibition
Assay Principle
Assay Performance Time
2h
Standard
1000 μg/mL
Sample Type
Serum, plasma, erythrocyte lysates and other biological fluids
Detection Range
15.63-1000 μg/mL
Sensitivity
5.7 μg/mL
Available Sizes
Frequently Asked Questions
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