Human AEA (Anandamide) ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AEA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AEA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AEA in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
N-Arachidonoylethanolamine
Reactivity
Human
Field of Research
Neuro science; Immunity
Assay Type
Competitive Inhibition
Assay Principle
Assay Performance Time
2h
Standard
200 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
3.13-200 ng/mL
Sensitivity
0.9 ng/mL
Available Sizes
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