Rat CR (Calretinin) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CR in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
CALB2; CAL2; CAB29; Calbindin 2,29kDa
Gene Aliases
CALB2; CAL2; CAB29; Calbindin 2,29kDa;
UniProt
P47728
Reactivity
Rat
Field of Research
Tumor immunity, Neuro science
Assay Type
Sandwich
Assay Principle
Assay Performance Time
3.5 H
Standard
50 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.79 - 50 ng/mL
Sensitivity
0.27 ng/mL
Product Datasheet
https://www.elkbiotech.com/upload/file/ELISA/ELK8988-1.pdf
Species
Rat
CAS Number
7732-18-5
Available Sizes
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