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Human TCPa (Alpha-Tocopherol) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TCPa protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TCPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TCPa in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

VE; Vitamin E; α-TCP; α-Tocopherol

Reactivity

Human

Field of Research

Metabolic pathway; Nutrition metabolism

Assay Type

Competitive Inhibition

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TCPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TCPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TCPa in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

2h

Standard

100 μg/mL

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.57-100 μg/mL

Sensitivity

0.47 μg/mL

Available Sizes

Curated Selection

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