Sheep NKB (Neurokinin B) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep NKB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep NKB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep NKB in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

NK-B; TAC3; NKNB; ZNEUROK1; Neuromedin K; Tachykinin 3; Neurokinin Beta

Reactivity

Sheep

Field of Research

Neuro science; Hormone metabolism

Assay Type

Competitive Inhibition

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep NKB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep NKB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep NKB in the samples is then determined by comparing the OD of the samples to the standard curve.