Anti-LAMP1 Antibody

Mouse Monoclonal Antibody specific to LAMP1 Antibody

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

LAMP-1, Lysosome-associated membrane protein 1, 120 kDa lysosomal membrane glycoprotein, LGP-120, CD107 antigen-like family member A, CD antigen CD107a

Gene Name

Lamp1

NCBI Gene ID

UniProt

P14562

Cellular Locus

Cell membrane, Endosome membrane, Lysosome membrane, Late endosome membrane, Cytolytic granule membrane

Host

Mouse

Reactivity

Rat, Hamster

Immunogen

Rat liver lysosome membranes.

Target Antigen

Lysosome-associated membrane glycoprotein 1

Target

LAMP1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IP

Field of Research

Cell adhesion

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, autophagy, and cholesterol homeostasis (By similarity). Plays also an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis (By similarity). {UniProtKB:P11279, UniProtKB:P11438}.

Additionnal Information

Immunoblotting: Use at 1ug/mL. A band of ~120kDa is detected. Immunocytochemistry: Use at 5-10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, rat, and hamster LAMP1.Accession no.: NP_036989.1 Gene ID: 25328

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Lysosome-associated membrane proteins (LAMP1 and LAMP2) are major constituents of the lysosomal membrane. The structures of these two proteins are closely related with 37% sequence homology. Both are transmembrane glycoproteins localized primarily in lysosomes and late endosomes. Cell surface LAMP1 and LAMP2 promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium which suggests that they are involved in adhesion of PBMC at sites of inflammation.