Caspase-8 (p18) Recombinant Rabbit Monoclonal Antibody [PSH05-83]
Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/CD95 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-325', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Caspase-8 CASP-8 Caspase-8 subunit p18 Casp8
Swiss Prot
O89110 Mouse
Cellular Locus
Cytoplasm. Nucleus.
Host
Rabbit
Species Reactivity
Mouse
Immunogen
Synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.
Isotype
IgG
Clone
PSH05-83
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IF-Cell: 1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 55 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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